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Outside the urinary bladders green arrows , fluorescence was observed at its highest concentration in the kidneys red arrows , lymph sacs draining the lower abdomen white arrows , and uterus blue arrow. Treatment with SXR activators increases expression of p53 and p53 target genes. The experiment was repeated twice in triplicates. Activation of the steroid and xenobiotic receptor, SXR, induces apoptosis in breast cancer cells. Activated SXR is necessary and sufficient to inhibit the growth of breast cancer cells. VEGF receptors and neuropilins are expressed in the urothelial and neuronal cells in normal mouse urinary bladder and are upregulated in inflammation. Western Blot, Flow Cytometry, Software.

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Treatment with SXR activators increases expression of p53 and p53 target genes.

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The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis doi: Data quantified by AlphaEase FC software are shown at the bottom of each panel. C SXR activation causes p53 accumulation. The knockdown efficiency was measured by measuring mRNA A or protein levels of p53 B after 48 and 72 hours of transfection respectively.

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The cells transfected with a non-specific scrambled siRNA or un-transfected cells were used as a control. All experiments were repeated at least three times. VEGF receptors and neuropilins are expressed in the urothelial and neuronal cells in normal mouse urinary bladder and are upregulated in inflammation.

Mice were fed a low-chlorophyll diet for 2 wk to reduce autofluorescence in the intestinal region and the abdominal hair was removed. Briefly, images were first acquired and stored with AlphaEase FC bit software Alpha Innotech and, subsequently Adobe Photoshop CS3 extended that permitted the determination of integrated density.

Image Quantitation and Protein, RNA & DNA Gel Quantitation

These results were replicated in at least three independent experiments. VEGF receptors and neuropilins are expressed in the urothelial and neuronal cells in normal mouse urinary bladder and are upregulated in inflammation doi: Western Blot, Flow Cytometry, Software.

The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis. Activation of the steroid and xenobiotic receptor, SXR, induces apoptosis in breast cancer cells doi: Outside the urinary bladders green arrowsfluorescence was observed at its highest concentration in the kidneys red softwadelymph sacs draining the lower abdomen white arrowsand uterus blue arrow.

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The experiment was repeated twice in triplicates. Cell Biosciences Inc alphaease fc software AlphaEase software.

SXR induced apoptosis is p53 dependent. Activated SXR is necessary and sufficient to inhibit the growth of breast cancer cells.

Activation of the steroid and xenobiotic receptor, SXR, induces apoptosis in breast cancer cells. The experiment was done in duplicates and the numbers represent the average from two independent runs.

PAR2-AP chronic treatment group.

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Cell proliferation was measured by fluorescence assay as above and the experiment was repeated at least twice.

The blot was probed with anti-SPP1 antibody. Camptothecin CAMP 24 hour treatment was used as a positive control for p53 induction.